Usage of EST data for non-model species in proteomics
Posted 30 September 2009 - 02:05 AM
I am working on two dimensional gel electrophoresis-based proteomics on a non-model species (i.e. genome not sequenced).
Recently, a team generated the cDNA library of my studied species. May I know if I can use these EST information to help identifiy the proteins that could not be matched after MALDI TOF/TOF?
Thank you, appreciated.
Posted 30 September 2009 - 03:45 PM
Posted 01 October 2009 - 07:09 AM
Posted 01 October 2009 - 09:05 PM
Sure, there shouldn't be a problem with it, be aware that RNA doesn't always equal protein though.
May I know what program should I use in order to carry out the search?