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pSecTag Expression of a protein with Signal Anchor


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#1 Benita

Benita

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Posted 29 September 2009 - 07:01 AM

Hey all,

this is my first question in this Forum. So maybe one of you "protein-guys" can help me.

I need a soluble Form of a Protein. It's a type II membrane protein. I've cloned the extracellular chain AND the signal anchor-sequence in the pSecTag-vector. So now I realized, that maybe now the protein will be anchored in the cytoplasmatic membrane, is this true? Or will it be secreted at all because of secretory vector?

Thank you for your help,

Franzi!

P.S.: I will use HEK cells as expression system.

Edited by Benita, 29 September 2009 - 07:01 AM.





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