Posted 29 September 2009 - 05:03 AM
Posted 29 September 2009 - 10:03 AM
if you were staining for protein (coomassie, india ink, etc) then the entire membrane will stain if you did.
if immunostaining then the entire membrane will stain if you didn't.
genius does what it must
i do what i get paid to do
Posted 12 October 2009 - 12:32 AM
Posted 26 October 2009 - 11:08 PM
if your prootein is stuk between the resolving and stacking, are u sure you are using the right percentage of gel??!!! try staining the gel and confirming if the protein is there. If that is the case may be your conditions need to be more appropriate. Try using methanol in the transfer buffer if you aint!!!