I'll be doing a high throughput screening using a 384-well microplate from thermo scientific. I'm generally quite new in molecular biology, so there are a number of things I need help with. I will be using p-nitrophenyl butyrate (PNPB) as a substrate for PHB depolymerase. Here are my questions:
1) What's the total volume for each well of the microplate?
2) What's the usual volume of transformed cells do you use for each well? (I understand that it should be 1 cell/well)
3) How do I solubilize PNPB? Is it in phosphate buffer?
4) Is the usual concentration used for PNPB, 0.5mM? How do I achieve that, if I'd like to make 10ml from the powdered PNPB with a MW of 209.2?
Thank you in advance for reading this and for your help!
0
No replies to this topic
