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Enhancement of homologous recombination in bacteria


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#1 sifa

sifa

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Posted 29 September 2009 - 01:11 AM

Hi everyone,

does anyone know how to enhance the last homologous recombination step in bacteria? Iīve the worst recombination rate ever. I even didnīt use any dilution steps after the overnight incubation before i platted the cells on the selective media (Chloramphenicol, because i used a marker replacement strategy and saccharose to get rid of the plasmid).
Any tipps like rising/lowering incubation temperature?

Thanks in advance
Sifa

:D




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