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Source of DNA for a Reporter Construct

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#1 myo-fusion



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Posted 28 September 2009 - 08:44 PM

I am planning to make my first luciferase reporter construct using the promoter region of my chosen gene. What would be the best source to PCR that region for cloning? If I need the promoter to be of human origin, can I just take a human cell line, isolate DNA and PCR it? Any tips for DNA isolation and PCR in this case?
Thank you!

#2 milla



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Posted 29 September 2009 - 12:36 PM

I did something similar.
I bought human genomic DNA from Biochain, but I think also extraction from cell line should work. Qiagen for sure has a kit for the DNA extraction.

Good luck!

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