Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Source of DNA for a Reporter Construct


  • Please log in to reply
1 reply to this topic

#1 myo-fusion

myo-fusion

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 28 September 2009 - 08:44 PM

I am planning to make my first luciferase reporter construct using the promoter region of my chosen gene. What would be the best source to PCR that region for cloning? If I need the promoter to be of human origin, can I just take a human cell line, isolate DNA and PCR it? Any tips for DNA isolation and PCR in this case?
Thank you!

#2 milla

milla

    member

  • Active Members
  • Pip
  • 21 posts
-1
Neutral

Posted 29 September 2009 - 12:36 PM

Hi,
I did something similar.
I bought human genomic DNA from Biochain, but I think also extraction from cell line should work. Qiagen for sure has a kit for the DNA extraction.

Good luck!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.