Posted 28 September 2009 - 12:41 PM
Posted 29 September 2009 - 05:43 AM
Posted 02 October 2009 - 08:12 AM
They do need to be at a specific OD but that will be different depending on what bacterium you're making competent. They need to be in log phase. If it's E coli that will be ~0.5 - my way of determining this is when you can only vaguely see the lab light thru the culture!
Posted 05 October 2009 - 08:34 PM
sometimes i just took an overnight, diluted 100 fold in fresh LB and let it grow for 2-3 hours.
Never let me down.