Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
* * * * * 1 votes

Detach and resuspend after centrifugation


  • Please log in to reply
No replies to this topic

#1 Me-Ka

Me-Ka

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 28 September 2009 - 08:22 AM

Hi,

To harvest bacteria (P. aeruginosa or S. marcescens ) I centrifuge them, but after centrifugation they agglomerate at the bottom of tube and attach to the wall. What is the best method to detach and resuspend them? I vortex (~1500 rpm) them now? Is it a good solution or it is destructive? Based on my experimental procedure, I should use them as soon as possible after harvesting.

Thanks for your help,




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.