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Detach and resuspend after centrifugation

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#1 Me-Ka



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Posted 28 September 2009 - 08:22 AM


To harvest bacteria (P. aeruginosa or S. marcescens ) I centrifuge them, but after centrifugation they agglomerate at the bottom of tube and attach to the wall. What is the best method to detach and resuspend them? I vortex (~1500 rpm) them now? Is it a good solution or it is destructive? Based on my experimental procedure, I should use them as soon as possible after harvesting.

Thanks for your help,

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