does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ
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#2
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Posted 28 September 2009 - 06:42 AM
newbie101, on Sep 28 2009, 10:15 AM, said:
does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ
Thymus, smooth muscle, kidney, spleen, adrenal cortex etc., but no skeletal muscle line that I know of.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
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Posted 06 October 2009 - 04:32 AM
Dr Teeth, on Sep 28 2009, 07:42 AM, said:
newbie101, on Sep 28 2009, 10:15 AM, said:
does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ
Thymus, smooth muscle, kidney, spleen, adrenal cortex etc., but no skeletal muscle line that I know of.
thats the thing..guess i have to make my own primary cultures then. do you know how long does a primary culture could last? i knew that its life span is much shorter compared to using cell lines
#4
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Posted 06 October 2009 - 04:45 AM
newbie101, on Oct 6 2009, 08:32 AM, said:
Dr Teeth, on Sep 28 2009, 07:42 AM, said:
newbie101, on Sep 28 2009, 10:15 AM, said:
does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ
Thymus, smooth muscle, kidney, spleen, adrenal cortex etc., but no skeletal muscle line that I know of.
thats the thing..guess i have to make my own primary cultures then. do you know how long does a primary culture could last? i knew that its life span is much shorter compared to using cell lines
A primary culture usually won't last through more than a handful of splittings, but are useful in that they tend to give more physiologic results since the lines aren't cancerous, polyploid, etc. However, if you wish to use this line for an extended period, you many be better off isolating your bovine skeletal muscle cells and transforming them into an immortalized culture line with SV40 or by other means.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
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Posted 08 October 2009 - 02:32 PM
newbie101, on Oct 6 2009, 11:32 PM, said:
Dr Teeth, on Sep 28 2009, 07:42 AM, said:
newbie101, on Sep 28 2009, 10:15 AM, said:
does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ
Thymus, smooth muscle, kidney, spleen, adrenal cortex etc., but no skeletal muscle line that I know of.
thats the thing..guess i have to make my own primary cultures then. do you know how long does a primary culture could last? i knew that its life span is much shorter compared to using cell lines
I would imagine that the structure of a skeletal fibre (ie fused myoblasts) would make culturing extremely difficult. The closest I’ve seen to culturing is a student who dissected out individual fibres and kept them alive for a day or so for experimenting.
