4 replies to this topic

[Kami23]

Hey all,

We are having a debate in the office about which is the correct term for sections?  Would they still be in vivo or are they now classed as ex vivo/in vitro?

[eberthella]

What do you mean by "sections"?   Seems in vitro and ex vivo are pretty inconsistent with in vivo.

[Kami23]

eberthella, on Sep 28 2009, 12:37 PM, said:

What do you mean by "sections"?   Seems in vitro and ex vivo are pretty inconsistent with in vivo.

ok for example sections for immunohistchemistry work.

[Dr Teeth]

Kami23, on Sep 28 2009, 08:01 AM, said:

eberthella, on Sep 28 2009, 12:37 PM, said:

What do you mean by "sections"?   Seems in vitro and ex vivo are pretty inconsistent with in vivo.

ok for example sections for immunohistchemistry work.

It seems to me that since the tissue is sectioned and experimented upon outside of the animal, ex vivo should be used.  In contrast, fluorescence detection methods used to visualize protein expression within a living animal should be in vivo. Nevertheless, every paper I have read that describes sectioned tissue immunohistochemistry uses in vivo, rather than ex vivo.

[Kami23]

Dr Teeth, on Sep 28 2009, 01:10 PM, said:

Kami23, on Sep 28 2009, 08:01 AM, said:

eberthella, on Sep 28 2009, 12:37 PM, said:

What do you mean by "sections"?   Seems in vitro and ex vivo are pretty inconsistent with in vivo.

ok for example sections for immunohistchemistry work.

It seems to me that since the tissue is sectioned and experimented upon outside of the animal, ex vivo should be used.  In contrast, fluorescence detection methods used to visualize protein expression within a living animal should be in vivo. Nevertheless, every paper I have read that describes sectioned tissue immunohistochemistry uses in vivo, rather than ex vivo.

Thanks Dr Teeth that helps loads!