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Fast protocol for RNA agarose gel electrophoresis


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#1 LabDiagMol

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Posted 28 September 2009 - 12:32 AM

Hi everyone,
I need to run my RNA samples on a denaturing agarose gel to check their integrity.
Could anyone tell me a fast protocol to do it?
Must be every component RNase free?

Thanks

#2 bob1

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Posted 28 September 2009 - 03:43 PM

Fast protocol is not to run denaturing, just ordinary agarose gel electrophoresis. If you are not recovering your RNA from the gel then things don't need to be ultra clean, just take ordinary molecular biology precautions such as not using spatulas in chemicals and using fresh buffers. I would use SB buffer rather than TBE or TAE so you can run it quickly, which means less chance of degradation. Be aware that RNA ladders designed for denaturing gels won't run true to size on non-denaturing gels.




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