how to know a sequene for a specific gene & produce it
Posted 27 September 2009 - 03:35 PM
we have a human gene of 500 bp which is responsible for production of a specific antibody for a certain infection
1. to know its sequence
2. produce it commercially as a pure human antibody to use it as treatment for this infection .
what are the techniques i have to use to achieve this goals .
in detail plz as much as u can
many thanks for every 1 thinking to help me.
Posted 27 September 2009 - 05:23 PM
If you know the gene, you can find the sequence through Pubmed. Then you need to make primers and clone the gene (from mRNA, I suspect). Let me know when you've done that much...
Posted 27 September 2009 - 11:31 PM
My bid is 10%
Posted 28 September 2009 - 01:59 PM
K BUT U SHOULD BE SURE OF UR ANSWER 1ST BUDDY SWANNY
THE PROBLEM IS THAT I DONT KNOW ANY THING ABT THIS GENE
SO HOW CAN I KNOW THE SEQUENCE??????????????
30% IF U CAN HELP ME B THIS LOOOOOOOOOOOOOL
I DON NEED UR DISCOUNT CAUSE I NEED DA ANSWER BEFORE THE NEGOTIATIONS GLEB KUDR.
Posted 28 September 2009 - 10:03 PM
First of all, please stop shouting at us (ie take the caps lock off...).
Second, your initial post said that you "had" a gene. That kind of suggests you know something about it. If you don't want to totally publicise the gene, send me a PM and we can talk further.
Posted 29 September 2009 - 02:03 AM
Than design primers and PCR it (from RNA template I suppose)
Than cloning into simple vector and sequencing to be sure it's target gene and not broken clone.
Than cut&paste into transmissible/production vector
..(transformation, obtaining stable protein accumulation and so on)..
Edited by gleb.kudr, 29 September 2009 - 02:04 AM.