Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to know a sequene for a specific gene & produce it


  • Please log in to reply
5 replies to this topic

#1 D LOFI

D LOFI

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 27 September 2009 - 03:35 PM

hello ev 1

we have a human gene of 500 bp which is responsible for production of a specific antibody for a certain infection

we need
1. to know its sequence
2. produce it commercially as a pure human antibody to use it as treatment for this infection .

what are the techniques i have to use to achieve this goals .
in detail plz as much as u can


many thanks for every 1 thinking to help me.

D lofi

#2 swanny

swanny

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 368 posts
10
Good

Posted 27 September 2009 - 05:23 PM

Give me 25% share in your product and I'll be glad to help... ;-0

If you know the gene, you can find the sequence through Pubmed. Then you need to make primers and clone the gene (from mRNA, I suspect). Let me know when you've done that much...
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#3 gleb.kudr

gleb.kudr

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
0
Neutral

Posted 27 September 2009 - 11:31 PM

Give me 25% share in your product and I'll be glad to help... ;-0


My bid is 10% ;)

#4 D LOFI

D LOFI

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 28 September 2009 - 01:59 PM

Give me 25% share in your product and I'll be glad to help... ;-0


K BUT U SHOULD BE SURE OF UR ANSWER 1ST BUDDY SWANNY

THE PROBLEM IS THAT I DONT KNOW ANY THING ABT THIS GENE
SO HOW CAN I KNOW THE SEQUENCE??????????????
30% IF U CAN HELP ME B THIS LOOOOOOOOOOOOOL


I DON NEED UR DISCOUNT CAUSE I NEED DA ANSWER BEFORE THE NEGOTIATIONS GLEB KUDR.

#5 swanny

swanny

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 368 posts
10
Good

Posted 28 September 2009 - 10:03 PM

Hey LOFI,
First of all, please stop shouting at us (ie take the caps lock off...).

Second, your initial post said that you "had" a gene. That kind of suggests you know something about it. If you don't want to totally publicise the gene, send me a PM and we can talk further.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#6 gleb.kudr

gleb.kudr

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
0
Neutral

Posted 29 September 2009 - 02:03 AM

Human genome are sequenced than you have a gene> you know its sequence.

Than design primers and PCR it (from RNA template I suppose)

Than cloning into simple vector and sequencing to be sure it's target gene and not broken clone.

Than cut&paste into transmissible/production vector

..(transformation, obtaining stable protein accumulation and so on)..

Profit!

Edited by gleb.kudr, 29 September 2009 - 02:04 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.