CellSpecific.com, on Sep 27 2009, 06:47 AM, said:
Your values are within a similar magnitude and so the minor difference might just reflect error inherent in technique. Can you tell us the volumes used during dilution and your colony counts per dilution.
hi, i used
doubling dilutions (1/2, 1/4/ 1/8, 1/16, 1/32) to obtain the turbidity readings. to do this i added 3 mL Luria broth and 3mL of Bacteria successively.
decimal dilutions (10^-6, 10^-7, 10^-8) to pour plate and count the colonies. on to the final plate, i added 1mL of 10^-6 for 10^-6, 0.1mL of 10^-7 and 1mL of 10^-8 to obtain 10^-6, 10^-7, 10^-8 final plate dilutions, respectively.
so this kind of discrepancy is normal i assume? as long as they are in the same magnitude it is acceptable?
which method would you guys say is more reliable?