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bacterial stock preparation


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3 replies to this topic

#1 sara.r

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Posted 24 September 2009 - 10:49 PM

most labs use 15-40 percent glycerol medium for bacterial stock preparation in -70 degree but is there any protocol for stock preparation in
-20 degree? how long are bacteria stable in this condition?
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#2 Chimp

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Posted 25 September 2009 - 02:23 AM

I would definitely use a -70 to -80 freezer. I think survival is prolonged a great deal.

#3 caren

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Posted 25 September 2009 - 04:37 AM

most labs use 15-40 percent glycerol medium for bacterial stock preparation in -70 degree but is there any protocol for stock preparation in
-20 degree? how long are bacteria stable in this condition?
best



I used to store completent in -20 degree ~3month. It is WORK.
In -70 degree ~1years.

#4 caren

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Posted 25 September 2009 - 04:40 AM

most labs use 15-40 percent glycerol medium for bacterial stock preparation in -70 degree but is there any protocol for stock preparation in
-20 degree? how long are bacteria stable in this condition?
best



I used to store completent in -20 degree ~3month. It is WORK.
In -70 degree ~1years.



Oh!! I forgot I use 10%glycerol to store them.




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