2 replies to this topic

[glycodog]

We are looking to isolate genomic DNA from human blood samples (200ul) and perform downstream sequencing reactions.  Does anyone out there have any ideas of a reliable miniprep kit?

Looking at

Qiagen- QIAamp DNA Blood Mini Kit
Invitrogen - Dynabeads® DNA DIRECT™ Blood  


Thanks

Glycodog

[LabDiagMol]

glycodog, on Sep 24 2009, 06:10 PM, said:

We are looking to isolate genomic DNA from human blood samples (200ul) and perform downstream sequencing reactions.  Does anyone out there have any ideas of a reliable miniprep kit?

Looking at

Qiagen- QIAamp DNA Blood Mini Kit
Invitrogen - Dynabeads® DNA DIRECT™ Blood  


Thanks

Glycodog

We are currently using Roche - High Pure PCR Template Preparation Kit for diagnostic procedures. It is very stable (batch to batch) and yields DNA good enough (purity and concentration) to perform downstream PCR or sequencing reactions. Miniprep kits are not souitable for Southern-blot.

Regards

[Kristina @Invitrogen Dynal]

Dynabeads DNA Direct Blood is a good alternative if you want to quickly isolate DNA for PCR reactions.

I think a better alternative for your sequencing application though would be our new DNA isolation technology which has a much higher sensitivity and yield: Dynabeads SILANE Genomic DNA.
This kit is fully scalable and from 200 µl human blood you can expect to isolate 5-6 µg of high quality gDNA.
More information on Dynabeads SILANE  here:

Kristina