Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Direct PCR (PCR without DNA extraction)


  • Please log in to reply
2 replies to this topic

#1 miso

miso

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 23 September 2009 - 09:38 PM

Hi people,
I have one question about Direct PCR. I want to ask if anybody using direct PCR for SNP detection but without commercial kit. I tried to boil samples 15 minute at 100 C and than i used in my PCR but i did not any results of my PCR product. I used cells from buccal swabs. I know that is some a commercial kits for direct PCR but I want to try this method without this kits and I want to request anybody for any simple guidance. Thank you very much for your help.

Miso :unsure:

#2 gleb.kudr

gleb.kudr

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
0
Neutral

Posted 26 September 2009 - 08:04 AM

Try to boil samples 5 minutes in 0.2M NaOH.

This technique is reported to be useful on plants and their cell walls are much stronger than human so I guess it may working.

http://www.ingentaco...000002/05112630

#3 miso

miso

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 27 September 2009 - 09:12 PM

Try to boil samples 5 minutes in 0.2M NaOH.

This technique is reported to be useful on plants and their cell walls are much stronger than human so I guess it may working.

http://www.ingentaco...000002/05112630


Thank you for your help. I will be try this idea. Thank




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.