Direct PCR (PCR without DNA extraction)
Posted 23 September 2009 - 09:38 PM
I have one question about Direct PCR. I want to ask if anybody using direct PCR for SNP detection but without commercial kit. I tried to boil samples 15 minute at 100 C and than i used in my PCR but i did not any results of my PCR product. I used cells from buccal swabs. I know that is some a commercial kits for direct PCR but I want to try this method without this kits and I want to request anybody for any simple guidance. Thank you very much for your help.
Posted 27 September 2009 - 09:12 PM
Try to boil samples 5 minutes in 0.2M NaOH.
This technique is reported to be useful on plants and their cell walls are much stronger than human so I guess it may working.
Thank you for your help. I will be try this idea. Thank