Posted 23 September 2009 - 08:54 AM
Posted 23 September 2009 - 10:50 AM
Edited by Dr Teeth, 23 September 2009 - 10:52 AM.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 23 September 2009 - 11:04 AM
Dilute some of the vector to 1-2 ng/ul and transform in competent cells and perform a maxiprep. When setting up your culture for the maxiprep, follow the appropriate protocol based on the plamid copy number. Confirm the identity of your plasmid by restriction digestions and sequencing! Many labs have sent me plasmids that were not what they were supposed to be or had mutations. To save yourself time, energy, and resources, SEQUENCE any plasmid sent to you by anyone before you test it experiementally!