5 replies to this topic

[planktonica]

Hi,

I am doing an experiment that requires a similar cell number per ml (about 1 million). I have no experience with this bacteria and don't have a standard curve to correlate OD with cell number.

I can't use a serial dilution and plating system because I need this cell culture.

Can somebody help me figuring out how to come up with this number/ml?

Thanks a lot!!

[gebirgsziege]

make a suspension in an appropriate liquid, count in haemocytometer, calculate cellnumber/mL, dilute (or add cells) so you get the cell number you need

[Chimp]

See if you can find something on the web! If no data exist for your particular bacterium, use data from another of similar size. These things usually don´t have to be extremely accurate anyway

[planktonica]

Thanks for the suggestions!

I've been looking for things on the web without much success

[pito]

planktonica, on Sep 23 2009, 06:51 PM, said:

Hi,

I am doing an experiment that requires a similar cell number per ml (about 1 million). I have no experience with this bacteria and don't have a standard curve to correlate OD with cell number.

I can't use a serial dilution and plating system because I need this cell culture.

Can somebody help me figuring out how to come up with this number/ml?

Thanks a lot!!

You could use a "system" like alamarblue to count and make a growth curve...and recalculate the total amount of numbers with a counting chamber (you do not know living from dead cells then... )

or do as gebirgsziege said.

[eberthella]

I'd not accept "something on the web."    Generate your own data.