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staph cell count


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5 replies to this topic

#1 planktonica

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Posted 23 September 2009 - 08:51 AM

Hi,

I am doing an experiment that requires a similar cell number per ml (about 1 million). I have no experience with this bacteria and don't have a standard curve to correlate OD with cell number.

I can't use a serial dilution and plating system because I need this cell culture.

Can somebody help me figuring out how to come up with this number/ml?

Thanks a lot!!

#2 gebirgsziege

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Posted 23 September 2009 - 11:01 PM

make a suspension in an appropriate liquid, count in haemocytometer, calculate cellnumber/mL, dilute (or add cells) so you get the cell number you need
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#3 Chimp

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Posted 25 September 2009 - 02:41 AM

See if you can find something on the web! If no data exist for your particular bacterium, use data from another of similar size. These things usually donīt have to be extremely accurate anyway

#4 planktonica

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Posted 25 September 2009 - 05:44 PM

Thanks for the suggestions!

I've been looking for things on the web without much success :(

#5 pito

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Posted 26 September 2009 - 07:29 AM

Hi,

I am doing an experiment that requires a similar cell number per ml (about 1 million). I have no experience with this bacteria and don't have a standard curve to correlate OD with cell number.

I can't use a serial dilution and plating system because I need this cell culture.

Can somebody help me figuring out how to come up with this number/ml?

Thanks a lot!!


You could use a "system" like alamarblue to count and make a growth curve...and recalculate the total amount of numbers with a counting chamber (you do not know living from dead cells then... )

or do as gebirgsziege said.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#6 eberthella

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Posted 27 September 2009 - 06:51 AM

I'd not accept "something on the web." Generate your own data.




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