Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

DNA concentration and Genome


  • Please log in to reply
1 reply to this topic

#1 The Question

The Question

    member

  • Active Members
  • Pip
  • 23 posts
0
Neutral

Posted 22 September 2009 - 08:04 AM

Can any one help how to convert the concentration of DNA (e.g. 464 ng/ul) to amount of genome (or DNA base pairs)? For example, I've a C elegans DNA concentration of 464 ng/ul. Now I want to know the amount of genome present in the solution.
We can find answers following our own ideas.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 4,432 posts
234
Excellent

Posted 22 September 2009 - 04:15 PM

c=n/v and n=mass/molar mass should do you.  For this you will need to know the molar mass of the genome which can probably be estimated by the number of bases in it and average nucleotide use.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.