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how stable is alpha mouse HRP?


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#1 planktonica

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Posted 21 September 2009 - 12:28 PM

Maybe this is just a cry for hope.
I was working VERY LATE friday night on a western blot and as I was tired and really wanted to leave the building, so I totally forgot to put he a-mouse-HRP back in the fridge and was sitting on the bench until today that I noticed it.

After kicking myself several times. I wonder.....

Is there any chance for this to be still usable? I think is not, but I wanted to ask, just in case.

Thanks.

#2 bob1

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Posted 21 September 2009 - 04:52 PM

The antibody may be OK, but the HRP is likely to be dead. You could test it, but the general rule for lab stuff is "If in doubt, throw it out".

Edited by bob1, 21 September 2009 - 04:52 PM.


#3 planktonica

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Posted 22 September 2009 - 03:04 PM

The antibody may be OK, but the HRP is likely to be dead. You could test it, but the general rule for lab stuff is "If in doubt, throw it out".


Thanks.
:P




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