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Single-cell RT-PCR with only ~10-100 Cells


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#1 anonymous

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Posted 19 September 2001 - 09:00 PM

Hi Mathias,

the RNeasy Kit from Qiagen is supposed to yield enough RNA from about 100 cellsGood luck


#2 Mathias Droll

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Posted 19 September 2001 - 09:00 PM

hi,i must identify mRNA from small frozen Tissue Samples isolated by Laser Microsdissection.I need a protocol of isolation RNA (-->RT-PCR) from frozen Tissue Samples. The samples contain only 10- 100cells!!!!!

#3 anonymous

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Posted 05 October 2001 - 09:00 PM

Maybe you could see "RT-PCR WITHOUT RNA ISOLATION", Klebe et al, BioTechniques 21: 1094-1100(Dec 1996). They report successful RT-PCR from less than 250 cells.




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