Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

coating meat tissue to ELISA plates


  • Please log in to reply
3 replies to this topic

#1 newbie101

newbie101

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 21 September 2009 - 04:42 AM

Hi,

Does anyone have any idea on how to coat ELISA plates with meat tissues?

TQ :angry:

#2 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 21 September 2009 - 05:29 AM

Almost everyone uses a "meat tissue" for example BSA...bovine serum albumin for blocking their plates. Could you be more specific?

#3 newbie101

newbie101

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 21 September 2009 - 06:06 PM

owh sorry about that. i should have been more specific. what i'm interested with is on how to coat bovine muscle and adipose tissue on the plates. the purpose of this step is not as blocking procedures but i will be looking at attachment studies on this type of tissues later on.

#4 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 22 September 2009 - 02:51 AM

I would use purified proteins for coating the plastic and block with BSA. You could then introduce your ligand incubate; wash and then use your detection system.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.