3 replies to this topic

[newbie101]

Hi,

Does anyone have any idea on how to coat ELISA plates with meat tissues?

TQ

[sgt4boston]

Almost everyone uses a "meat tissue" for example BSA...bovine serum albumin for blocking their plates.  Could you be more specific?

[newbie101]

owh sorry about that. i should have been more specific. what i'm interested with is on how to coat bovine muscle  and adipose tissue on the plates. the purpose of this step is not as blocking procedures but i will be looking at attachment studies on this type of tissues later on.

[sgt4boston]

I would use purified proteins for coating the plastic and block with BSA.  You could then introduce your ligand incubate; wash and then use your detection system.