1 reply to this topic

[Sonia]

I run a 2-step RT-PCR by using reverse primer in reverse transcription, and then add equal amount of both reverse and forward primers in PCR.

Does anyone can tell me there are 2 bands in the lanes or a band smear?

please see the attachment

Thank you

Attached Thumbnails

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[gleb.kudr]

Sonia, on Sep 20 2009, 04:13 AM, said:

I run a 2-step RT-PCR by using reverse primer in reverse transcription, and then add equal amount of both reverse and forward primers in PCR.

Does anyone can tell me there are 2 bands in the lanes or a band smear?

Looks like smear for me.