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2 bands or smear? 2-step RT-PCR


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#1 Sonia

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Posted 20 September 2009 - 04:13 AM

I run a 2-step RT-PCR by using reverse primer in reverse transcription, and then add equal amount of both reverse and forward primers in PCR.

Does anyone can tell me there are 2 bands in the lanes or a band smear?

please see the attachment

Thank you

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  • N190909.jpg


#2 gleb.kudr

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Posted 20 September 2009 - 06:37 AM

I run a 2-step RT-PCR by using reverse primer in reverse transcription, and then add equal amount of both reverse and forward primers in PCR.

Does anyone can tell me there are 2 bands in the lanes or a band smear?


Looks like smear for me.




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