3 replies to this topic

[borriello.87]

Hi,
I'm going to stimulate human CD4 T lymphocyte with PMA/Iono for intracellular cytokine staining
and I have some questions:

1) What concentrations of PMA and Iono do I have to use?
2) Is it enough to stimulate cells for 4 hours?
3) Do I have to add Bleferdin A at the beginning or in the middle of stimulation?

Thank you!!!

[CellSpecific.com]

This paper will tell you how:

J Exp Med. 2007 January 22; 204(1): 65–71

[borriello.87]

Dear Jay,
Thank you for your answer!
I have another question for you...Do you know any other way to stimulate CD4 T lymphocytes for intracellular cytokine staining besides PMA/Iono?

[CellSpecific.com]

PMA/IONO is the quick and easy way. You can use anti-CD3 activation methods, but your signals might not be strong enough depending on the type of cells you have. You'll have to run preliminary tests to be sure.

Edited by Jay Dela Cruz PhD, 24 September 2009 - 05:01 AM.