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PMA/Iono


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#1 borriello.87

borriello.87

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Posted 19 September 2009 - 04:45 AM

Hi,
I'm going to stimulate human CD4 T lymphocyte with PMA/Iono for intracellular cytokine staining
and I have some questions:

1) What concentrations of PMA and Iono do I have to use?
2) Is it enough to stimulate cells for 4 hours?
3) Do I have to add Bleferdin A at the beginning or in the middle of stimulation?

Thank you!!!

#2 borriello.87

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Posted 22 September 2009 - 09:27 AM

Dear Jay,
Thank you for your answer!
I have another question for you...Do you know any other way to stimulate CD4 T lymphocytes for intracellular cytokine staining besides PMA/Iono?




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