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[metta]

hi everybody,

i'm using gamma-irradiated MEF as feeder layer for culturing ES cells. The MEF looks normal (with monolayer) on 6 well and 10cm plate. However, we see some morphological structures similar to small ES colonies when we place them on a 96-well plate (from falcon and greiner).


are there any other people have similar issues with MEF grown on 96 well plate or any suggestions how to deal with this.

thanks
m