what is the point of serial dilution???
Posted 17 September 2009 - 09:05 PM
I am working on transfection in order to gain stable cells.
According to the method that I have, 24 hours after transfection of DNA to HeLa cell, I am supposed to perform serial dilution and wait for another 24 hours for cells to be stablized.
Here, I don't get why it needs serial dilution.
Thx a loooooooooot in advance!!
Posted 18 September 2009 - 07:06 AM
are you asking about serial dilution of the cells or serial dilution of the selection agent (antibiotic)?
If you are talking about the selection agent, you do a serial dilution when you do not know the correct concentration of agent to effectively kill off the untransfected cells while keeping your positive cells alive. You only need to do this once in order to determine the concentration to use in subsequent transfections.
I hope this helps,
Posted 20 September 2009 - 05:02 PM