Uneven leveling in resolving gel
Posted 17 September 2009 - 11:28 AM
Posted 20 September 2009 - 05:13 PM
Posted 23 September 2009 - 12:08 PM
Try overlaying with water saturated butanol and make sure you clean your plates well with water and ethanol.
Is it ok if I substitute water saturated butanol with water saturated isopropanol? And does the alcohol actually help to level the gel or does it only get rid of the bubbles?
Posted 23 September 2009 - 04:17 PM
Posted 29 September 2009 - 04:19 PM
No, water is miscible with propanol but not with butanol - if you use propanol you will dehydrate your gel and it won't fix your leveling problems.
I tried what you suggested. I clean the plates thoroughly with ddH20 and ethanol. I then tried overlaying the gel with water saturated butanol. Though at first it appears as if the gel is fine, once the gel polymerizes the leveling is awkward.
Am I doing something wrong while pouring the gel? Should I overlay the water saturated butanol slowly? Or is my gel recipe not quite right for the 10% gel? Any help is greatly appreciated. I am going crazy trying to figure out where I am going wrong. I use the Bio-Rad mini protean cell setup to cast my gel.
Posted 06 October 2009 - 10:27 PM
Posted 08 October 2009 - 06:05 PM