4 replies to this topic

[Biobaba]

Hello there..

I have recently started working with the cancer cell line MDA-MB-175. I am involved in testing anti-cancer activity of certain antibodies against this cell line and I have troubles in having them in culture for my assays. They seem to grow very slow! It takes almost a week for me to do the next passage/ sub culture and hence the assay is delayed too! I am using standard DMEM F12 medium with 2mM L-glutamine and 10% FCS suppliments.

Is there anything I can do to make them grow more quick!!

cheers

[Stephan]

Try seeding the population at a higher density. Also try increasing the FBS to 20% in the beginning.
Sometimes cells grow slow for the first few subcultures.

[bob1]

IIRC these do grow quite slowly and have problems with contact inhibition. Make sure they are not too confluent when passaged.

[Biobaba]

Thank you stefan and bob I shall try your suggestions!

cheers!

[Biobaba]

sorry about the typo stephan!