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MDA-MB-175


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#1 Biobaba

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Posted 17 September 2009 - 06:35 AM

Hello there..

I have recently started working with the cancer cell line MDA-MB-175. I am involved in testing anti-cancer activity of certain antibodies against this cell line and I have troubles in having them in culture for my assays. They seem to grow very slow! It takes almost a week for me to do the next passage/ sub culture and hence the assay is delayed too! I am using standard DMEM F12 medium with 2mM L-glutamine and 10% FCS suppliments.

Is there anything I can do to make them grow more quick!!

cheers

#2 Stephan

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Posted 18 September 2009 - 12:17 AM

Try seeding the population at a higher density. Also try increasing the FBS to 20% in the beginning.
Sometimes cells grow slow for the first few subcultures.

#3 bob1

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Posted 20 September 2009 - 04:56 PM

IIRC these do grow quite slowly and have problems with contact inhibition. Make sure they are not too confluent when passaged.

#4 Biobaba

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Posted 20 September 2009 - 11:27 PM

Thank you stefan and bob I shall try your suggestions!

cheers!

#5 Biobaba

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Posted 20 September 2009 - 11:28 PM

sorry about the typo stephan!




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