Posted 17 September 2009 - 06:35 AM
I have recently started working with the cancer cell line MDA-MB-175. I am involved in testing anti-cancer activity of certain antibodies against this cell line and I have troubles in having them in culture for my assays. They seem to grow very slow! It takes almost a week for me to do the next passage/ sub culture and hence the assay is delayed too! I am using standard DMEM F12 medium with 2mM L-glutamine and 10% FCS suppliments.
Is there anything I can do to make them grow more quick!!
Posted 18 September 2009 - 12:17 AM
Sometimes cells grow slow for the first few subcultures.
Posted 20 September 2009 - 04:56 PM
Posted 20 September 2009 - 11:27 PM