Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Problems with Phusion Polymerase (Finnzymes)


  • Please log in to reply
3 replies to this topic

#1 JinalB

JinalB

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 September 2009 - 06:04 AM

Our lab has optimized a PCR to amplify the envelope gene of HIV-1 cDNA from first round single genome amplification using the Phusion polymerase (Finnzymes). This was a guarenteed PCR that up until recently never gave any problems. We've tried to change the protocol, but nothing has helped. We've tried amplifying old samples that had previously been ampified with the Phusion polymerase, and these too have failed.

Has anyone else experienced problems with this enzyme on routine PCRs?

#2 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 17 September 2009 - 06:11 AM

Nope....this enzyme works fine for my applications. But I am up to ordering new enzyme so I am interested in this. Something similar happened here with a cloning kit....we got replacements two times but they changed maufacturer of something in the kit. When they returned to the old one it started working again.

Have you contacted Finnzymes if something is wrong with the lot?
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)

#3 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 652 posts
53
Excellent

Posted 17 September 2009 - 06:51 PM

I haven't had probs with Phusion either, and we have just got a new aliquot which is working as normal.

Edited by leelee, 17 September 2009 - 06:52 PM.


#4 JinalB

JinalB

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 September 2009 - 10:24 PM

Nope....this enzyme works fine for my applications. But I am up to ordering new enzyme so I am interested in this. Something similar happened here with a cloning kit....we got replacements two times but they changed maufacturer of something in the kit. When they returned to the old one it started working again.

Have you contacted Finnzymes if something is wrong with the lot?


The new enzymes we've used are Phusion lots 37 and 40. We've also ordered from two different suppliers, and we're waiting for a response from them as to whether they've altered any of the components. So we just thought we'd check on the net to see if anyone else was having similar problems in the interim.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.