Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene (capillary based air-heating real-time thermal cycler)???
Thks!
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Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene
Started by LabDiagMol, Sep 17 2009 02:28 AM
2 replies to this topic
#2
gleb.kudr
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Posted 17 September 2009 - 01:29 PM
LabDiagMol, on Sep 17 2009, 02:28 AM, said:
Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene (capillary based air-heating real-time thermal cycler)???
Thks!
Thks!
Not used but what a problem? Any RT-cycler will be fine.
#3
LabDiagMol
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Posted 18 September 2009 - 03:21 AM
Two main differences:
- Surface -->glass (capillary) or plastic (plates)
- Glass capillary-based RT-cyclers heats samples by air convection and plate-based RT-cyclers heats by peltier system. The kinetics of heating/cooling processes are very different.
Most probe manufacturers do not guarantee a good performance under these conditions specially if they only produce plate-based RT-cyclers (Applied Biosystems)
Physics and busssines are sometimes unpredictable!
- Surface -->glass (capillary) or plastic (plates)
- Glass capillary-based RT-cyclers heats samples by air convection and plate-based RT-cyclers heats by peltier system. The kinetics of heating/cooling processes are very different.
Most probe manufacturers do not guarantee a good performance under these conditions specially if they only produce plate-based RT-cyclers (Applied Biosystems)
Physics and busssines are sometimes unpredictable!
