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Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene


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#1 LabDiagMol

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Posted 17 September 2009 - 02:28 AM

Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene (capillary based air-heating real-time thermal cycler)???

Thks!

#2 gleb.kudr

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Posted 17 September 2009 - 01:29 PM

Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene (capillary based air-heating real-time thermal cycler)???

Thks!


Not used but what a problem? Any RT-cycler will be fine.

#3 LabDiagMol

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Posted 18 September 2009 - 03:21 AM

Two main differences:
- Surface -->glass (capillary) or plastic (plates)
- Glass capillary-based RT-cyclers heats samples by air convection and plate-based RT-cyclers heats by peltier system. The kinetics of heating/cooling processes are very different.
Most probe manufacturers do not guarantee a good performance under these conditions specially if they only produce plate-based RT-cyclers (Applied Biosystems)
Physics and busssines are sometimes unpredictable!




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