Hello,
can someone please help out, I suddenly have started getting many bands in my SDS-PAGE after staining and destaining. I made sure that my comb was clean and my gel plates were properly washed and cleaned but I still got these bands after running SDS-PAGE analysis. Previously I got only bands for my target protein but now I have these unwanted bands.
Please any suggestions about how to get rid of these unwanted bands?
Thank in advance
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5 replies to this topic
#2
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Hmmm, I think it's working
Posted 20 September 2009 - 05:11 PM
Gels run using whole cell lysate? purified protein?
#3
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an elder
Posted 24 September 2009 - 10:35 AM
one of your buffers is probably contaminated.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#5
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member
Posted 06 October 2009 - 10:18 PM
One possible reason is sample degradation.
Or, did you change the voltage from the power supply? If you use a lower voltage, it is possbile that the previously unresolved bands are separated (low V, high resolution).
Or, did you change the voltage from the power supply? If you use a lower voltage, it is possbile that the previously unresolved bands are separated (low V, high resolution).
#6
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Veteran
Posted 07 October 2009 - 05:03 AM
Hi ojbillions, welcome to the BioForums!
To answer your question, we need more information about your procedure. When you say "bands", do you mean bands on a stained PAGE gel or bands on a western blot of a PAGE gel? What is the nature of the sample you're loading -- is it a whole cell lysate or a purified protein sample?
To answer your question, we need more information about your procedure. When you say "bands", do you mean bands on a stained PAGE gel or bands on a western blot of a PAGE gel? What is the nature of the sample you're loading -- is it a whole cell lysate or a purified protein sample?
