1 reply to this topic

[tivnana]

Hi,

I am "thinking" about trying to generate a cell flask which will contain a mixture of HEK293cells and Kelly (NBL,neuroblastoma cells). I firstly want to try and get the two of them to grow in the same media type...either convert one to DMEM or the other to RPMI. If I can get over this first hurdle has anyone else ever tried this?

The reason is because I want to test a neuroblastoma specific delivery mechanism and it would be nice if I had a mixed population of neuroblastoma and "normal" cell lines growing. Successful delivery would result in just the NBL cells lighting up.

Has anyone any experience with this? Am I just being optimistic about this working? Should I just resort to two wells, one 293 and the other Kelly?

All help would be greatly appreciated,

Thanks.

[bob1]

Unless the cells proliferate at the same rate and aren't contact inhibited you are likely to have problems with one cell line out-competing the other over time. There shouldn't be a problem with growing both in the same flask for a short time though.