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protein expression


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#16 ram

ram

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Posted 05 November 2009 - 08:06 AM

I forgot to write and observed it for the 3rd time that although there in no difference in protein profiles, the growth (as reflected in the turbidity and size of the pellet after harvesting) in induced culture is always less then that in uninduced . Why is it so? Does IPTG retard growth rate?



I haven't observed so in my case....but if it is so, it could be because, the entire biosynthetic machinery in the bacteria will be switched off (or in a low mode) to divert resources for expressing the foreign gene in excess.....this might affect the growth rate.........


Thats what I also had thought of initially, but if would have been the case, there should have been the clear difference in the protein profiles which is not the case! I really wonder :D
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.




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