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DMMB dye for GAG measurement in chondrogenesis


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1 reply to this topic

#1 tzyy yue

tzyy yue

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Posted 15 September 2009 - 06:00 PM

hi everyone,

i need a bit of help :(
i have been trying to track the level of chondrogenesis by measurement of GAG level
in the cells. i`ve read about the DMMB method, but.... but.... i found it hard
to get more recent information regarding the DMMB method,
all the things i have read seem to be old, i`d like to ask if anyone
know about the DMMB method? is it still being performed?

if i use a layer of GAG coating upon the culture dish, can i add DMMB into the medium to detect the GAG level?
can the GAG in the cells be secreted and released into the medium?

pls--- thnks a lot!! :(

yue

#2 nicilaw

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Posted 13 April 2010 - 06:49 AM

hi everyone,

i need a bit of help ;)
i have been trying to track the level of chondrogenesis by measurement of GAG level
in the cells. i`ve read about the DMMB method, but.... but.... i found it hard
to get more recent information regarding the DMMB method,
all the things i have read seem to be old, i`d like to ask if anyone
know about the DMMB method? is it still being performed?

if i use a layer of GAG coating upon the culture dish, can i add DMMB into the medium to detect the GAG level?
can the GAG in the cells be secreted and released into the medium?

pls--- thnks a lot!! ;)

yue


Although this answer comes a little late, maybe it can help someone else:
A KIT using this method (really comfortable):
http://www.biocolor....noglycan-assay/

If the KIT is to expensive for you, use this paper:
"Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies"
Isabelle Barbosa, Stéphanie Garcia, Véronique Barbier-Chassefière, Jean-Pierre Caruelle, Isabelle Martelly and Dulce Papy-García
http://glycob.oxford...t/full/13/9/647

Have fun ;-)




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