Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cytoplasmic/nuclear fraction


  • Please log in to reply
1 reply to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 193 posts
2
Neutral

Posted 15 September 2009 - 09:00 AM

HI

I did some cytoplasmic and nuclear protein extration. After which I ran a western on both cytoplasmic and nuclear fraction to check for beta actin and histone expression (loading controlz). I get even loading for beta actin in the cytoplasmic and nuclear fraction and equal loading for histone in the cytoplasmic and nuclear fraction. Does this mean contamination? Is beta actin only to be detceted in the cytoplasmic fraction and is histone only to be detected in the nuclear fraction?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,242 posts
336
Excellent

Posted 15 September 2009 - 04:42 PM

Histones are only found in the nucleus, but actin is found in both.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.