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Please Help! Low intensity of Annexin V in Flow cytometry


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#1 Ambinlab

Ambinlab

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Posted 15 September 2009 - 08:22 AM

:( Dear All,
I am in trouble. There is only 5 tests left and my annexin V FITC kit seems to be expired!
I obtain very low signal of Annexin V FITC and quite good signal of PI. Is there any trick so I can gain some results from the remaining samples?? Should I change the binding buffer or use it more concentrated?
in trouble please help:(:(:(
Regards




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