Uneven bands in SDS-PAGE
Posted 14 September 2009 - 03:26 PM
I am using gels purchased from BioRad, and fresh running and laemmli buffer. This has happened more than once, so I doubt it is an issue with the gel itself.
Any ideas on whats going on and how to fix it?
Posted 14 September 2009 - 05:36 PM
Edited by bob1, 14 September 2009 - 05:37 PM.
Posted 14 September 2009 - 08:16 PM
Posted 15 September 2009 - 02:15 PM
During loading, I have been careful to not push into the well. I have the tip in the middle and load, letting the sample sink to the bottom.
If there is salt in my samples, I have no clue where it is coming from. I do a protein precipitation before using Calbiochems proteoextract kit, then resolubilize the proteins in loading buffer. According to calbiochem, there should not be anything in the protein pellet wash solution that would interfere with running the a 1D SDS-PAGE (in case my pellet is not totally dry before resolubilizing it)
Posted 17 September 2009 - 04:12 AM