2 replies to this topic

[scienceyay]

Hi guys, I had a bit of a mishap with my real-time instrument, basically the computer couldn't find it.  I had made a master mix for 48 samples (with primer)...as you know not particularly cheap.  When I couldn't get the run on I just froze everything at -20.  I was wondering if I can still use the mastermix or will "something?!" have happened in the tube.  Can't think of anything apart from maybe somekind of primer dimer?


Cheers

[phage434]

It should work fine.  Next time, I'd probably store it at 4C, which would not freeze and might better protect the enzyme.

[gleb.kudr]

I'd rather add some polymerase cause it may be damaged on -20C without glycerin protection.