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Freezing primers+SYBR green


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#1 scienceyay

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Posted 14 September 2009 - 07:48 AM

Hi guys, I had a bit of a mishap with my real-time instrument, basically the computer couldn't find it. I had made a master mix for 48 samples (with primer)...as you know not particularly cheap. When I couldn't get the run on I just froze everything at -20. I was wondering if I can still use the mastermix or will "something?!" have happened in the tube. Can't think of anything apart from maybe somekind of primer dimer?


Cheers :)

#2 phage434

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Posted 16 September 2009 - 06:36 PM

It should work fine. Next time, I'd probably store it at 4C, which would not freeze and might better protect the enzyme.

#3 gleb.kudr

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Posted 17 September 2009 - 02:15 PM

I'd rather add some polymerase cause it may be damaged on -20C without glycerin protection.




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