Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Nonradiactive labelling of probes

  • Please log in to reply
1 reply to this topic

#1 labguy



  • Members
  • Pip
  • 1 posts

Posted 14 September 2009 - 04:42 AM

I need help to find a good (and cheap) labelling kit or method for labelling DNA-probes with a non radioactive label. The probe will be used both for screening colony blots as well as probe in hybridisation to Southern blots. Im working at at lab with no equipment for film development, so I guess it must be a detection system based on colour development on the filter?
Kit name/producer or reference to method will be highly appreciated.
Any suggestions ?

#2 hobglobin


    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,604 posts

Posted 14 September 2009 - 06:59 AM

Digoxigenin (DIG) and Biotin-labeling comes to my mind. Google them, don't know if they are fitting to your further protocols...

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.