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Nonradiactive labelling of probes


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#1 labguy

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Posted 14 September 2009 - 04:42 AM

Hi
I need help to find a good (and cheap) labelling kit or method for labelling DNA-probes with a non radioactive label. The probe will be used both for screening colony blots as well as probe in hybridisation to Southern blots. Im working at at lab with no equipment for film development, so I guess it must be a detection system based on colour development on the filter?
Kit name/producer or reference to method will be highly appreciated.
Any suggestions ?

#2 hobglobin

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Posted 14 September 2009 - 06:59 AM

Digoxigenin (DIG) and Biotin-labeling comes to my mind. Google them, don't know if they are fitting to your further protocols...

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