SDS-PAGE, please help!!!
Posted 04 September 2001 - 09:00 PM
Posted 05 September 2001 - 09:00 PM
Though I am not an expert, I have heard of instances where proteins do not migrate according to theirestimate size on small gels (10 cm). I believe that a larger gel apparatus with a longer run time would give better resolution. However, if bovine trypsin migrates as expected, then I am at a loss. One other idea would be to try a different gel system. Novex/Invitrogen has a large selection of gels designed for smaller proteins. I think you might have luck with tricine gels. Good luck!
Posted 06 September 2001 - 09:00 PM