Accidently heated up the wrong DNA. Damage?
Posted 14 September 2009 - 03:09 AM
Instead of the sample tubes containing plasmid DNA to be digested (to check insert), I've placed my actual plasmid DNA stock itself in the 37OC! It was there for ten minutes - will it be okay?
Posted 14 September 2009 - 03:25 AM
Run a sample out on a gel and check.
Posted 16 September 2009 - 05:40 AM
It depends on how clean your DNA prep is. The heat itself will not damage the DNA
BTW if you place your plasmid DNA to 94C for 5 minutes it will be mess.