Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer extension


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 12 October 2001 - 09:00 PM

I am having trouble with primer extension. The only product we are getting are bands as if they were primer-dimers. Does anyone have experience with this and what to do to get rid of it. Also we have tried between 10and 20ug Total RNA and I am wondering if this is enough. On a Northern we see a strong signal with 10ug total RNA. tHANKYOU

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 25 November 2001 - 10:00 PM

since primer dimers formed in your experiment, why does you not redesign new one instead of old one? 10-20ug total RNA is appropriate. alternatively you can use mRNA for primer extension.

I am performing primer extension too now but still have no outcome.share your experience when you success.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.