Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cell isolation


  • Please log in to reply
1 reply to this topic

#1 peanuts

peanuts

    member

  • Active Members
  • Pip
  • 12 posts
1
Neutral

Posted 11 September 2009 - 03:32 PM

I wonder is there anyone has experience on epithelial cell isolation (enterocytes)?
I am currently doing some isolation works from fish, but the viability and purity are quite varied every time.
Please leave a message if you have experience on this (any animals).

Thanks!

#2 Kristina @Invitrogen Dynal

Kristina @Invitrogen Dynal

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 21 September 2009 - 01:04 AM

Hi

What kind of isolation strategy are you using at the moment? What are the yield and purities you are able to achieve?
Dynabeads are widely used for epithelial cell isolation from several species. Providing there exists a good antibody against your fish enterocytes, then this could perhaps be a strategy to consider? I would be happy to send you a suggested protocol if you are interested.

Kind Regards

Kristina




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.