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SY5Y protein content


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#1 hlod

hlod

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Posted 10 September 2009 - 03:06 PM

Hello,

Does anyone have a rough estimate protein yield for SY5Y? I've been lysing it from 10cm dishes at around 5x10^6 cells /dish in 1ml of RIPA, and got only 250ug by BCA.

Is my lysis method inefficient or do I simply need to lyse in less buffer?

Thanks




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