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Oligo storage


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#1 almost a doctor

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Posted 10 September 2009 - 01:46 AM

Hi, does anyone know how long can oligos be kept at 4C for?

I've just been rummaging the fridge and found some oligos ordered by a former colleague that left about a year ago. I've already thrown quite a few of them, but there are some labelled ones that I know where a bit pricey so thought better to double check.
Can I freeze them now, or should I not bother and just chuck them?

Thanks :)

#2 phage434

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Posted 10 September 2009 - 04:02 AM

I'd guess they were ok, if you still have a use for them. This is why TE rather than water should be used to dissolve oligos, since in TE they would almost certainly be ok.

#3 Adrian K

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Posted 13 September 2009 - 09:39 PM

I used to mistaken put my stock oligos on benches for a month without realising it (i store in tips box) and I still be able to use it in my pcr. However, it is not recommended.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

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#4 almost a doctor

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Posted 14 September 2009 - 04:36 AM

Thanks guys. I think the primers were reconstituted in TE so hopefully they'll be fine. I've put them at -20C will find out if they are okay next time someone needs them. Thing is, not sure anyone is going to use them anytime soon, but it was a shame to throw them.

Cheers again :)




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