Ca treatment to cells
Posted 09 September 2009 - 09:50 AM
can anyone pls help....calcium is precipitating out when used at higher conc as Cacl2 to culture .....not in the lower calcium conc
Is it because of pH?...I dont use CaP04 transfection....and this Ca treatment is given only in DMEM+LG media and Ca shows precipitates in 5-10 mins
Posted 09 September 2009 - 01:07 PM
Posted 10 September 2009 - 10:10 PM
Ya I am facing this problem....at these 2 ph it precipitates....can u pls tell what shud i do? bcoz the ph of the DMEM gradually increases as usage.shall I decrease the Co2 levels of incubator or what?