Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

eluting antigen


  • Please log in to reply
No replies to this topic

#1 dnafactory

dnafactory

    member

  • Active Members
  • Pip
  • 16 posts
0
Neutral

Posted 09 September 2009 - 07:11 AM

Dear all,
after trying so many times to couple the antibody to the beads, it looks like I managed (though I must improve it!) but when I elute with 0,1M Glycine pH 2,5 my antibody breaks down and I get the Fab fraction into the gel. I suppose it's the Fab because I can't detect it for western but I see it by colloidal staining.
I would like to elute with a competing peptide but I could't find a good protocol or guidelines for designing the peptide (single peptide? tandem repeats of the peptide? and how many?). Can you help me, please?
Thanks!!!!
DNAfactory




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.