Dear all,
after trying so many times to couple the antibody to the beads, it looks like I managed (though I must improve it!) but when I elute with 0,1M Glycine pH 2,5 my antibody breaks down and I get the Fab fraction into the gel. I suppose it's the Fab because I can't detect it for western but I see it by colloidal staining.
I would like to elute with a competing peptide but I could't find a good protocol or guidelines for designing the peptide (single peptide? tandem repeats of the peptide? and how many?). Can you help me, please?
Thanks!!!!
DNAfactory
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