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increase the amount of cDNA


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#1 BioPe

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Posted 08 September 2009 - 12:06 PM

Hi!

Is it possible to produce more cDNA from cDNAs samples? I cannot reverse transcribe my RNA samples because they are over...

Thxs!

#2 Carlton H

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Posted 09 September 2009 - 09:06 AM

Isn't that what PCR is for? :lol:
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#3 Dr Teeth

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Posted 09 September 2009 - 10:13 AM

Hi!

Is it possible to produce more cDNA from cDNAs samples? I cannot reverse transcribe my RNA samples because they are over...

Thxs!


BD makes a cDNA amplification kit.
http://www.bdbioscie...amp;item=112351

Are you doing RACE?

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#4 Unagi

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Posted 19 September 2009 - 06:12 PM

You can, but you have to careful;
- If you re-input too much cDNA, it will inhibit your subsequent PCR
- cDNA contamination of the general working area can become a serious issue
- You may start to get non-specific products driven by the sequence homology and high concentration of the cDNA template (increasing the tm may help here).
- I would not rely on the second round of PCR for any quantitation.




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