Hi!
Is it possible to produce more cDNA from cDNAs samples? I cannot reverse transcribe my RNA samples because they are over...
Thxs!
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#2
Carlton H
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Posted 09 September 2009 - 09:06 AM
Isn't that what PCR is for? 
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Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!
If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
#3
Dr Teeth
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Posted 09 September 2009 - 10:13 AM
BioPe, on Sep 8 2009, 04:06 PM, said:
Hi!
Is it possible to produce more cDNA from cDNAs samples? I cannot reverse transcribe my RNA samples because they are over...
Thxs!
Is it possible to produce more cDNA from cDNAs samples? I cannot reverse transcribe my RNA samples because they are over...
Thxs!
BD makes a cDNA amplification kit.
http://www.bdbioscie...amp;item=112351
Are you doing RACE?
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#4
Unagi
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Posted 19 September 2009 - 06:12 PM
You can, but you have to careful;
- If you re-input too much cDNA, it will inhibit your subsequent PCR
- cDNA contamination of the general working area can become a serious issue
- You may start to get non-specific products driven by the sequence homology and high concentration of the cDNA template (increasing the tm may help here).
- I would not rely on the second round of PCR for any quantitation.
- If you re-input too much cDNA, it will inhibit your subsequent PCR
- cDNA contamination of the general working area can become a serious issue
- You may start to get non-specific products driven by the sequence homology and high concentration of the cDNA template (increasing the tm may help here).
- I would not rely on the second round of PCR for any quantitation.
