Posted 08 September 2009 - 10:53 AM
Posted 08 September 2009 - 11:01 AM
Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before adding the antibody and keep the cells after fixation with 4% PFA?
Yes, I keep them in 1x PBS at 4°C until ready for staining.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
Posted 08 September 2009 - 04:43 PM