Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before adding the antibody and keep the cells after fixation with 4% PFA?
Thanks
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#2
Dr Teeth
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Posted 08 September 2009 - 11:01 AM
Postdoc_G, on Sep 8 2009, 02:53 PM, said:
Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before adding the antibody and keep the cells after fixation with 4% PFA?
Thanks
Thanks
Yes, I keep them in 1x PBS at 4°C until ready for staining.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
#3
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 08 September 2009 - 04:43 PM
Don't keep them for too long though, a week or two maximum.
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miBunny
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Posted 08 September 2009 - 06:26 PM
And don't let them dry out!!!!
