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Immunocytochemistry


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#1 Postdoc_G

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Posted 08 September 2009 - 10:53 AM

Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before ‎adding the antibody and keep the cells after fixation with 4% PFA?‎
Thanks ‎

#2 Dr Teeth

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Posted 08 September 2009 - 11:01 AM

Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before ‎adding the antibody and keep the cells after fixation with 4% PFA?‎
Thanks ‎


Yes, I keep them in 1x PBS at 4C until ready for staining.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 bob1

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Posted 08 September 2009 - 04:43 PM

Don't keep them for too long though, a week or two maximum.

#4 miBunny

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Posted 08 September 2009 - 06:26 PM

And don't let them dry out!!!!




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